The Study of the Comparative Chemical Profile of Polar and Nonpolar Fractions of Cinnamomum Tamala Leaves by Chromatography and Spectroscopic Methods
DOI:
https://doi.org/10.64882/ijrt.v14.iS1.1050Keywords:
Cinnamomum tamala, HPLC, GC-MS, Thin layer chromatography, Eugenol, PhytochemicalsAbstract
Cinnamomum tamala (CT) is a member of the Lauraceae family, and its leaves are most popularly used as a spice in Indian food. Along with spice, it is associated with many medicinal values such as antioxidant, antidiabetic, antifungal, immunomodulation, etc. Here, we made a study about the qualitative analysis of different phytochemicals in different fractions of CT leaves, along with their TLC fingerprinting. Based on GC-MS analysis of polar and nonpolar fractions, Eugenol was reported as an active constituent that is present in excess in nonpolar fraction. Further, eugenol was analysed by High Performance Liquid Chromatography (HPLC) using a C-18 column. The results showed that the distribution of different phytochemicals varied among the different fractions, with the maximum amount of eugenol found in the non-polar fraction (Hexane fraction) and then in the total methanol fraction.
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