Synthesis and Biological Profiling of a Co(II) Complex Featuring 2-Acetoxybenzoic Acid as a Bidentate Ligand

Abstract

Aspirin, also known as acetylsalicylic acid, is considered the most effective and widely used anti-inflammatory drug which helps treat the majority of musculoskeletal and articular disorders. Novel Co (II) complex containing aspirin have been produced and characterized by utilizing infrared, melting point, and electrochemical studies (polarography and amperometry). The ligand has been discovered to have bidentate chelating properties. The produced compounds were formed as ML₂ based on the obtained analytical data. Aspirin binds to both oxygen of carboxyl groups. The complexes of metals were discovered to possess a range of inhibiting effects on microorganisms.

The present work the analysis of drugs and their modified forms using different physicochemical and electrochemical techniques. The Findings and Conclusion of present work is in two parts. Firstly, it attempts to investigate and developed Polarographic and Voltammetric methods for the analysis of such type of drug designing samples. On the basis of obtained results it could be concluded that Polarography has got some peculiar advantageous features and versatility. The Voltammeric and Polarographic methods have proved to be very powerful tools for the simultaneous determination of different origin samples like industrial use, pharmaceutical formulations, biological inorganic and organic compounds etc. Secondly design of modified anti-stroke medicines for better potency and safety for stroke problem.

Physiochemical, microbial and antiplatelet activity studies of 2-Acetoxy benzoic acid (Aspirin) and its complex with Co(II) have been done in aqueous and solid phase. Polarographic i.e. Direct current Polarography (DCP)& Differential Pulse Polarography (DPP) and Amperometric titration methods have been used for the qualitative and quantitative analysis of Aspirin and its complex with Co(II). Metal ligand interaction studied using Polarographic method at 25 ± 1⁰C and ionic strength of µ = 1.0 (Ammonium chloride Buffer).

On the basis of results of Polarograpy, Amperometry and IR Spectral studies the probable formula of Co(II) - Aspirin has been designed and stoichiometric ratio is 1:2 (M:L). The drug co-ordinated to metal through the 2 oxygen atom of carboxyl group thus the drug act as bidentate ligand, which is finding from IR spectral analysis data.

Microbial study of Co(II)- Aspirin complex has been done against various pathogenic bacteria viz. Staphylococcus aureus, Bacillus pumilus, Proteus mirabilis and Escherichia coli. Using Disc diffusion method. The results revealed that the complex is more potent as compared to the parent drug.

The evaluation of Antiplatelet activity of drug metal complex was carried out on human plasma through a platelet aggregation analyser. Arachidonic acid and ADP were used to gauge any changes in platelet shape and aggregation process. The parent drugs showed some antiplatelets activity, however their drug metal complexes demonstrated better efficacy.